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Gene delivery systems.
| Expression Type | Efficiency (%) | Cell Viability (%) | Safety | |
|---|---|---|---|---|
| Virus* | Stable, or Transient | 80–90% | 80–90% | Low |
| Electroporation | Transient | 50–70% | 40–50% | High |
| TF reagent ** | Transient | 20–30% | 80–90% | High |
Virus including adenovirus (transient), retrovirus (stable), and lentivirus (stable);
TF reagent, transfection reagents including PEI (Polysciences Inc.), FuGENE HD (Promega), and Lipofectamine 2000 (Invitrogen);
All values are ours (unpublished experiments).
