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. 2011 Jun 10;12(6):3831–3845. doi: 10.3390/ijms12063831

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© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Western blot assay of pro-caspase-3 and activated caspase-3 in K562 cells. β-actin was used as an internal control. Caspase-3 exists as its inactive form pro-caspase-3 and there are no detectable active caspase-3 subunits in K562 cells. Administration of tryptanthrin induces mito cyt-c leakage and activates pro-caspase-3. The expression of active caspase-3 subunits p20 and p17 were remarkably elevated while pro-caspase-3 contents decreased after tryptanthrin exposure. However, addition of a pan-caspase inhibitor ZVAD-FMK significantly attenuated the pro-caspase-3 activation in response to tryptanthrin. The contents of p20 and p17 remarkably decreased in the presence of ZVAD-FMK. (A) Lane 1: control; lane 2: 0.5%DMSO; lane 3: 6.25 μg/mL tryptanthrin; lane 4: 12.5 μg/mL tryptanthrin; lane 5: 25 μg/mL tryptanthrin; (B) Lane 1: control; lane 2: ZVAD-FMK; lane 3: ZVAD-FMK + 6.25 μg/mL tryptanthrin; lane 4: ZVAD-FMK + 12.5 μg/mL tryptanthrin; lane 5: ZVAD-FMK + 25 μg/mL tryptanthrin.