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Western blot assay of Bcl-2, Bax and β-actin protein expression in K562 cells. (A) expression of Bcl-2 and Bax after tryptanthrin exposure. β-actin was used for normalization and verification of protein loading; (B) the Bcl-2/Bax ratio in the control, 0.5% DMSO and each tryptanthrin-treated groups. Lane 1: control; lane 2: 0.5% DMSO; lane 3: 6.25 μg/mL tryptanthrin; lane 4: 12.5 μg/mL tryptanthrin; lane 5: 25 μg/mL tryptanthrin.
