Table 1.
Characteristics of the 20 microsatellite loci isolated from Thamnaconus modestus.
| Locus | Repeat Motif | Primer Sequence (5′→3′) | Ta (°C) | Genbank Accession No. |
|---|---|---|---|---|
| KTm138 | (CA)13-(CA)8 | F: CTAATTTCCCAAGGTAAGGTT 6-fam R: TCATTAAAAAGCCAATCAGAA |
60 | FJ210983 |
| KTm141 | (GT)12 | F: CGTGATGTCGCTGTAAGG hex R: TCTGGCAGTTTTTCTTTTTAT |
57 | FJ210984 |
| KTm151 | (GT)22 | F: CACAGACAGAAGGTCAGAGAG 6-fam R: ATTCATGCAACTTACACGACT |
55 | FJ210987 |
| KTm25 | (GT)6-(GT)5 | F: TGATTACCTCAAAACTTGTGT hex R: CCTTCAGCTGTAAATCTCATA |
57 | FJ210993 |
| KTm220 | (GT)7CT(GT)7AT(GT)10 | F: AGTTTTTAGATTTGCGGTTGT 6-fam R: AATTCCCCCTACAGTCTTCTC |
57 | FJ210997 |
| KTm222 | (GT)13 | F: TGGGTTTTGTTGGGTAA hex R: CCCCCTCTATTTAGATTATGT |
57 | FJ210999 |
| KTm223 | (CA)11CT(CA)9 | F: CTGCTGCACGTGTCTCA ned R: TGTGATAGCCAAAGTCTGATG |
57 | FJ211000 |
| KTm234 | (CA)14 | F: TTGCCTTTACAGTGACAAACA 6-fam R: GGATGGAGGGGAAGAGA |
63 | FJ211001 |
| KTm246 | (CA)12 | F: AAACGGCTCATGTTAATCTGT ned R: AATTCTGCAGCCGTTTAGAC |
63 | FJ211003 |
| KTm252 | (GT)24 | F: ACACGTTTGCAGATTTGTAAT hex R: GCCACTCTCTAGGGTAGTAGG |
57 | FJ211006 |
| KTm254 | (GT)13 | F: AGCCCTAATATAAACACACTG hex R: TGCGCAGGATACATTG |
52 | FJ211007 |
| KTm255 | (GT)11 | F: AGCGAAGAGAACATTCCTC ned R: CTGCCAAGATCCTAACTTTGT |
57 | FJ211008 |
| KTm261 | (GT)17 | F: TTCAGATTTGATTGTGAGGAT 6-fam R: TGAAGGGCAGACTTGTTTAC |
63 | FJ211010 |
| KTm268 | (GT)10 | F: ATTATCACCCCCACAAGTTCT ned R: CGGCTAAATCATGTTTCTGA |
60 | FJ211012 |
| KTm271 | (GT)16 | F: TGTGGTGTTTACTGCAGATAA hex R: ACAGGGTCATGAAAATAATGT |
57 | FJ211013 |
| KTm274 | (GT)20 | F: ATGGAAATAAGCCTCTTGTTC hex R: CTTCTGCAAACCTAAATCAAA |
57 | FJ211016 |
| KTm279 | (GT)5TT(GT)15 | F: AGTTTGACGGCTGACATTTAT hex R: AGCGCTTCAGATCAGATTCT |
57 | FJ211018 |
| KTm283 | (CA)35 | F: GATCTCCATCTCCACCTT 6-fam R: TTGGCTATTGGTAAATTATTC |
57 | FJ211019 |
| KTm286 | (CA)12 | F: GAACTGTGCAACTGTGTTTTC hex R: AAATGTCGCTGTATCTGCTG |
63 | FJ211021 |
| KTm292 | (GT)14 | F: ATCTGCCATTCACTTCCTTTA hex R: CGGAATGTGATCTGCTTTG |
51 | FJ211022 |
Ta is the optimal annealing temperature.