Table 2.
Population (No) | Microsatellite Loci
|
|||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KTm 138 |
KTm 141 |
KTm 151 |
KTm 25 |
KTm 220 |
KTm 222 |
KTm 223 |
KTm 234 |
KTm 246 |
KTm 252 |
KTm 254 |
KTm 255 |
KTm 261 |
KTm 268 |
KTm 271 |
KTm 274 |
KTm 279 |
KTm 283 |
KTm 286 |
KTm 292 |
Mean | ||
FST | −0.007 | 0.028 | 0.025 | 0.026 | 0.018 | 0.017 | 0.002 | −0.002 | 0.010 | −0.006 | 0.008 | −0.001 | 0.009 | 0.007 | −0.013 | 0.006 | −0.002 | 0.002 | −0.011 | −0.006 | 0.005 | |
Geoje Wild (60) | NA | 9 | 8 | 13 | 5 | 17 | 13 | 15 | 9 | 35 | 18 | 13 | 16 | 9 | 12 | 6 | 18 | 14 | 20 | 5 | 12 | 12.44 |
AR | 7.73 | 7.37 | 9.88 | 4.38 | 14.95 | 11.05 | 11.69 | 7.93 | 28.13 | 14.42 | 10.35 | 14.18 | 7.99 | 9.50 | 5.50 | 14.36 | 11.47 | 17.16 | 4.50 | 10.13 | 10.53 | |
S | 180–208 | 92–108 | 224–270 | 170–180 | 194–236 | 124–154 | 250–286 | 210–230 | 224–322 | 104–144 | 94–128 | 320–368 | 224–240 | 322–370 | 164–180 | 138–184 | 142–192 | 150–218 | 90–98 | 168–198 | ||
F | 0.442 | 0.375 | 0.342 | 0.800 | 0.175 | 0.258 | 0.408 | 0.342 | 0.067 | 0.175 | 0.333 | 0.258 | 0.433 | 0.333 | 0.533 | 0.183 | 0.300 | 0.175 | 0.400 | 0.225 | 0.32 | |
U | 3 | 1 | 4 | 1 | 5 | 3 | 5 | 4 | 18 | 4 | 3 | 5 | 2 | 5 | 2 | 4 | 6 | 4 | 0 | 3 | 3.4 | |
HE | 0.743 | 0.779 | 0.756 | 0.348 | 0.911 | 0.846 | 0.789 | 0.768 | 0.968 | 0.898 | 0.783 | 0.879 | 0.755 | 0.812 | 0.650 | 0.894 | 0.817 | 0.918 | 0.722 | 0.854 | 0.811 | |
Ho | 0.750 | 0.750 | 0.733 | 0.333 | 0.917 | 0.800 | 0.800 | 0.767 | 0.967 | 0.900 | 0.933 | 0.933 | 0.750 | 0.867 | 0.433 | 0.917 | 0.867 | 0.950 | 0.500 | 0.800 | 0.780 | |
FIS | −0.010 (0.990) | 0.037 (0.040) | 0.030 (0.044) | 0.043 (0.587) | −0.007 (0.131) | 0.055 (0.724) | −0.015 (0.501) | 0.001 (0.739) | 0.002 (0.525) | −0.002 (0.579) | −0.194 (0.005) | −0.063 (0.302) | 0.006 (0.740) | −0.068 (0.593) | 0.335 (0.000) | −0.025 (0.924) | −0.062 (0.043) | −0.035 (0.650) | 0.310 (0.000) | 0.064 (0.300) | ||
P | 0.984 | 0.039 | 0.064 | 0.621 | 0.127 | 0.661 | 0.541 | 0.698 | 0.600 | 0.547 | 0.008 | 0.304 | 0.756 | 0.639 | 0.000 | 0.903 | 0.033 | 0.683 | 0.000 | 0.352 | ||
Geoje Hatchery (30) | NA | 7 | 7 | 11 | 5 | 13 | 10 | 13 | 5 | 22 | 17 | 13 | 12 | 7 | 8 | 4 | 16 | 9 | 18 | 5 | 11 | 10.00 |
AR | 7.00 | 7.00 | 11.00 | 5.00 | 13.00 | 10.00 | 13.00 | 5.00 | 22.00 | 17.00 | 13.00 | 12.00 | 7.00 | 8.00 | 4.00 | 16.00 | 9.00 | 18.00 | 5.00 | 11.00 | 10.00 | |
S | 182–194 | 92–106 | 224–250 | 170–182 | 192–230 | 126–152 | 248–282 | 216–224 | 232–304 | 104–146 | 94–130 | 324–368 | 226–240 | 320–338 | 166–172 | 138–194 | 144–190 | 146–210 | 90–98 | 172–198 | ||
F | 0.500 | 0.433 | 0.233 | 0.917 | 0.167 | 0.333 | 0.367 | 0.383 | 0.117 | 0.200 | 0.250 | 0.317 | 0.433 | 0.233 | 0.567 | 0.200 | 0.367 | 0.150 | 0.417 | 0.250 | 0.33 | |
U | 1 | 0 | 2 | 1 | 1 | 0 | 3 | 0 | 5 | 3 | 3 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 0 | 2 | 1.0 | |
HE | 0.706 | 0.723 | 0.860 | 0.160 | 0.915 | 0.786 | 0.795 | 0.694 | 0.950 | 0.897 | 0.859 | 0.831 | 0.731 | 0.841 | 0.625 | 0.913 | 0.759 | 0.915 | 0.703 | 0.870 | 0.799 | |
Ho | 0.767 | 0.900 | 0.700 | 0.100 | 0.933 | 0.967 | 0.967 | 0.600 | 0.967 | 0.967 | 0.967 | 0.800 | 0.733 | 0.833 | 0.467 | 0.867 | 0.700 | 0.933 | 0.567 | 0.800 | 0.744 | |
FIS | −0.087 (0.635) | −0.250 (0.096) | 0.189 (0.090) | 0.381 (0.002) | −0.020 (0.431) | −0.235 (0.003) | −0.220 (0.480) | 0.138 (0.103) | −0.018 (0.603) | −0.080 (0.123) | −0.128 (0.116) | 0.038 (0.524) | −0.004 (0.902) | 0.009 (0.031) | 0.257 (0.187) | 0.052 (0.342) | 0.079 (0.674) | −0.021 (0.010) | 0.196 (0.158) | 0.082 | ||
P | 0.640 | 0.091 | 0.051 | 0.004 | 0.447 | 0.004 | 0.464 | 0.114 | 0.685 | 0.181 | 0.989 | 0.135 | 0.539 | 0.907 | 0.035 | 0.199 | 0.390 | 0.663 | 0.014 | 0.132 |
Single-locus FST, number of samples (No), number of alleles per locus (NA), allellic richness (AR), size in bp of alleles (S), frequency (F) of the most common allele, number of unique alleles (U), expected heterozygosity (HE), observed heterozygosity (Ho), inbreeding coefficient (FIS), and probability of significant deviation from Hardy-Weinberg equibrium after Bonferroni correction (P, initial α = 0.05/20 = 0.003) are given for each population and locus. Calculations assume that individuals with one microsatellite band are homozygous for the allele. Number in parenthesis below FIS indicates the probability of significant heterozygosity excess or deficit.