Table 2.

Bacterial strains, plasmids and oligonucleotide primers used in this study.

Strain or plasmid	Relevant characteristics	Reference
Rhizobium leguminosarumbv. trifolii
Rt24.2	Wild type, Rifr, Nxr	[36]
Rt2472	Rt24.2 derivative carrying mini-Tn5 between 151–152 bp position of rosR, Rifr, Kmr	[41]
ANU843	Wild type, Rifr	[57]
ANU851	ANU843 derivative carrying Tn5::nodD, Kmr	[57]
E. coli
VH1000	MG1655 derivative, lacZ, lacI, pyrE+	[58]
LG01	MG1655 derivative, phoB519::Tn5, Kmr	[59]
Plasmids
pUC19	Cloning and sequencing vector, Apr	[60]
pMP220	IncP, mob, promoterless lacZ, Tcr	[61]
pMJ221	pUC19 containing 126-bp EcoRI-XbaI fragment of the rosR upstream region (based on primers: pROS2/pREW2 and pRBAM1/pREW3)	This work
pMJ222	pUC19 containing 126-bp EcoRI-XbaI fragment of the rosR upstream region (based on primers: pROS2/pREW4 and pRBAM1/pREW3)	This work
pMJ223	pUC19 containing 126-bp EcoRI-XbaI fragment of the rosR upstream region (based on primers: pROS2/pREW1)	This work
pBR1	pBBR1MCS-2 containing 1100-bp EcoRI-BamHI fragment with rosR of Rt24.2	[41]
pEP1	pMP220 carrying the −403 to +243 bp fragment of the rosR coding region	[36]
pEP9	pMP220 carrying the −403 to −185 bp fragment of the rosR upstream region	[36]
pEP10	pMP220 carrying the −403 to −232 bp fragment of the rosR upstream region	[36]
pEP13	pMP220 carrying the −357 to −232 bp fragment of the rosR upstream region	[42]
pEP14	pMP220 carrying the −357 to −268 bp fragment of the rosR upstream region	[42]
pEP15	pMP220 carrying the −339 to −268 bp fragment of the rosR upstream region	[42]
pEP21	pMP220 containing 126-bp EcoRI-XbaI fragment from pMJ221	This work
pEP22	pMP220 containing 126-bp EcoRI-XbaI fragment from pMJ222	This work
pEP23	pMP220 containing 126-bp EcoRI-XbaI fragment from pMJ223	This work
Oligonucleotide primers	Sequence (5′→3′)*
pROS2	GAGCCCCTGAATTCTTCATCTGTCA	This work
pREW1	AGGGATCTAGAAGCAGTACGCTAGACATTCAC	This work
pREW2	GTAATTCGGATCCAGAACTCTACTTGCA	This work
pREW3	GAAATCAAAACTGAGGGATCTAGAAGCA	This work
pREW4	GTAATTCGGATCCCGAACTCTACTTGCA	This work
pRBAM1	AAATGCAAGTAGAGTTCTGGATCCGAATTA	This work

^*Sequences for EcoRI, BamHI and XbaI restriction sites are underlined.