Figure 4.
Fluorescence recovery traces with fitted curves and residuals from 8 of the spots photobleached in Figure 7. In some of the spots (a, b) there was appreciable photobleaching of fluorescence due to the low (but finite) intensity laser beam used for monitoring the fluorescence, while in others (f), there little photobleaching was evident. Table 3 reports the diffusion constant and mobility recovered from curve fitting procedures performed on the 8 curves.