Table 3.
Overview of cyclization products of squalene with A. acidocaldarius SHC muteins
| Mutation(s) | Product pattern | Reference(s) |
|---|---|---|
| Y606A/W23V/W495V/W522V/W533A/W591L/W78S/E35Q/E197Q/D530N/T378A | Same product pattern and activity as the wild type | 50, 84 |
| Y612F/D376E/D376G/D377E/D377G/D377Q/E45A/E45D/F365W/T41A/E93A/R127Q/W133A/Y267A/F434A/F437A/W258L/D350N/D421N/D442N/H451R/D447N/D377N/D313N/E535Q/D374E | Same product pattern as the wild type with less enzyme activity | 16, 24, 27, 83, 86 |
| D376E + D377E/D376Q/D376R/D377R/E45K/W406V/W417A/D377C + V380E + V381A/D376C + C435S/D374I/D374V/H451F | No enzyme activity | 16, 24, 47, 83 |
| F605A | hop-22(29)-ene; hopanol; podioda-8,17,21-triene; podioda-7,17,21-triene; 17-epi-dammara-20(21),24-diene; dammara-13(17),24-diene; eupha-7,24-diene; 20-hydroxy-17-epi-dammarene; hop-17(21)-ene; hop-(16)17-ene; neohop-13(18)-ene; neohop-12(13)-ene | 31 |
| Q262G/Q262A/P263G/P263A | hop-22(29)-ene; hopanol; hop-21(22)-ene | 86 |
| Y609Fa | α-polypodatetraene; dammara-13(17),24-diene; 17-isodammara-12,24-diene; eupha-7,24-diene; dammara-20(21),24-diene; 17-isodammara-20(21),24-diene; hopene; diaplopterol | 27 |
| D377C/D377N/Y612A | hop-(22)29-ene; hopanol; 3-deoxyhomologe of achilleol A | 37, 83 |
| F601A | hop-(22)29-ene; hopanol; 6-6-5-fused tricycles; 6-6-6-5-fused tetracycle | 37 |
| F605A | 6-6-5-fused tricyclic; 6-6-6-5-fused tetracyclic; 6-6-6-6-5-fused pentacyclic skeletons | 37 |
| F365A | 6-6-fused bicyclic skeleton | 37 |
| Y420A | 6-6-fused bicyclic; 6-6-5-fused tricyclic skeletons | 37 |
| Y609A/Y612A/L607K | 6-6-5-fused tricyclic skeleton | 37 |
| Y609Fa | 6-6-fused bicycle; 6-6-6-5-fused tetracyclic skeletons | 37 |
| I261A | 6-6-5-fused tricycle; 6-6-6-5-fused tetracycle; 6-6-6-6-5-fused pentacyclic skeletons | 37 |
| W169F/W169H/W489A/F605K | 6-6-6-5-fused tetracyclic skeleton | 37 |
a
Phenotype of Y609F mutein is contrarily described in two publications.