Table 1.

ORFs and gene products of GTE2

ORFa	aa (% identity)b	Protein function (motif)c	Matchd	E valuee
1	159 (42)	Unknown	VWBp32, Streptomyces phage	1e−22
2	560 (48)	Terminase (pfam03354)	SSPB78_13581, Streptomyces sp. strain SPB78	5e−154
3	477 (40)	Putative portal (pfam05133)	Hypothetical protein, Corynebacterium urealyticum DSM 7109	4e−81
4	235 (34)	Unknown	Gp5, Mycobacterium phage Ramsey	7e−23
5	154 (36)	Unknown	Hypothetical protein, Micrococcus luteus SK58	2e−07
6	322 (55)	Main capsid (pfam05065)	Putative structural phage protein, Corynebacterium urealyticum DSM 7109	2e−59
7	170 (40)	Unknown	Gp9, Mycobacterium phage Halo	2e−18
8	114 (31)	Unknown	Gp10, Mycobacterium phage Che9d	2e−04
9	91	Unknown
10	121 (29)	Unknown	Gp13, Mycobacterium phage Che9d	1e−07
11	282 (32)	Major tail (pfam05345)	RER_22600, Rhodococcus erythropolis PR4	6e−38
12	98 (38)	Unknown	MAB_1794, Mycobacterium abscessus ATCC 19977	6e−07
13	88 (48)	Unknown	Jden_2318, Jonesia denitrificans DSM 20603	3e−06
14	1548 (34)	Tape measure protein (COG5412)	Gp21, Mycobacterium phage CrimD	8e−46
15	309 (33)	Tail protein	Gp18, Mycobacterium phage Che9d	2e−34
16	532 (47)	Tail protein	Gp19, Mycobacterium phage Che9d	2e−142
17	130 (42)	Unknown (pfam10910)	Gp20, Mycobacterium phage Che9d	6e−19
18	182 (58)	Putative lysin	Nfa15420, Nocardia farcinica IFM 10152	3e−36
19	317 (49)	Unknown	Nfa15430, Nocardia farcinica IFM 10152	2e−71
20	163 (30)	Unknown	Nfa600, Nocardia farcinica IFM 10152	6e−09
21	103	Unknown
22	372 (26)	Unknown	Hypothetical protein, Rhodococcus equi ATCC 33707	7e−10
23	296 (59)	Tail fiber	gp4, Mycobacterium phage Bxb1	2e−16
24	213 (45)	Unknown	GTE5p025, Gordonia terrae phage GTE5	4e−12
25	214 (31)	Unknown	Namu_2691, Nakamurella multipartita DSM 44233	4e−11
26	193 (68)	Unknown (pfam10263)	TM4_gp80, Mycobacterium phage TM4	2e−51
27	211	Unknown
28	147	Unknown
29	130	Unknown
30	96 (42)	Archeal Holliday junction resolvase (pfam01870)	VRR-NUC domain-containing protein, Alkaliphilus oremlandii OhILAs	1e−12
31	181	Unknown
32	163 (33)	Unknown	Gp59, Mycobacterium phage PLot	4e−04
33	370 (26)	Unknown	Hypothetical protein, Desulfovibrio aespoeensis Aspo-2	2e−04
34	128	Unknown
35	796 (31)	DNA Pol I (COG0749)	DNA-directed DNA polymerase I, Chryseobacterium gleum ATCC 35910	8e−72
36	153 (43)	dCMP deaminase, putative (pfam00383)	dCMP deaminase, Archaeoglobus fulgidus DSM 4304	1e−29
37	280	Cutinase (pfam01083)
38	289 (30)	Thymidylate synthase (pfam00303)	Thymidylate synthase, Methanosaeta thermophila PT	2e−06
39	298	Unknown
40	82 (55)	Unknown	Hypothetical protein, Aeromicrobium marinum DSM 15272	8e−04
41	119 (44)	Phage-encoded dCTP pyrophosphatase (pfam08761)	Phage-encoded dCTP pyrophosphatase, Anoxybacillus flavithermus WK1	2e−06
42	171	Thymidine monophosphate kinase (TMPK) (cd01672)
43	107	Unknown
44	462 (30)	Helicase (Pfam00271, Pfam00176)	Hypothetical protein, Holdemania filiformis	1e−45
45	118	Unknown
46	148	Unknown
47	666 (23)	Putative primase (COG3598)	Gp46, Rhodococcus phage ReqiPine5	4e−10
48	128	Unknown
49	138	Unknown
50	98	Unknown
51	66	Unknown
52	63	Unknown
53	156	Unknown
54	139	Unknown
55	103	Unknown
56	121	Unknown
57	131 (39)	Homing endonuclease (pfam01844)	Gp49, Burkholderia phage KS9	8e−07

^aORFs were numbered consecutively.

^bThe percent identity was based on the best match when a BlastP analysis was performed. aa, amino acid.

^cPredicted function is based on amino acid identity, conserved motifs, N-terminal sequencing, and gene location within functional modules.

^dThe most closely related gene (only if named) and the name of the organism are given.

^eThe probability of obtaining a match by chance as determined by BLAST analysis. Only values less than 10⁻⁴ were considered significant.