Table 2.
LAMP and PCR primers used in this study to detect Salmonella by targeting the invA gene
| Primer name | Sequence (5′–3′) | Positiona | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| LAMP primers | ||||
| F3 | CGGCCCGATTTTCTCTGG | 503–520 | Ladder-like bands with variable sizes | This study |
| B3 | CGGCAATAGCGTCACCTT | 665–682 | ||
| FIP | GCGCGGCATCCGCATCAATA-TGCCCGGTAAACAGATGAGT | 573–592 (F1c), 527–546 (F2) | ||
| BIP | GCGAACGGCGAAGCGTACTG-TCGCACCGTCAAAGGAAC | 593–612 (B1c), 635–652 (B2) | ||
| Loop-F | GGCCTTCAAATCGGCATCAAT | 547–567 | ||
| Loop-B | GAAAGGGAAAGCCAGCTTTACG | 613–634 | ||
| PCR and qPCR primers | ||||
| 139 | GTGAAATTATCGCCACGTTCGGGCAA | 371–396 | 285 | 36 |
| 141 | TCATCGCACCGTCAAAGGAACC | 634–655 |
a
The positions are numbered based on the coding sequence of Salmonella serovar Typhimurium invA gene (GenBank accession number M90846).