Table 1.
Absorption and kinetic data of dyes reduced by BTI10
Substrate | λmax (nm) | ε (M−1·cm−1) | Vmax (μM·s−1·mg−1)b | Km (μM) | Vmax/Km |
---|---|---|---|---|---|
BHQ-10 | 515 | 35,600 | 290 | 59 | 4.9 |
Janus green B | 594a | 38,000 | ND | ND | ND |
Flame orange | 483 | 28,359 | 150 | 510 | 0.29 |
Methyl orange | 466 | 24,846 | 510 | 300 | 1.7 |
o-Methyl red | 435 | 23,400 | 130 | 9.7 | 13.5 |
Loss of absorbance was measured at 660 nm.
ND, not determined.