Table 4.
Persistence and stability of φRSL1 particles in plants and soil
| Plant or soila | Wt (g) | Mean phage titerb (PFU/g) ± SD |
|---|---|---|
| Plant 1 | ||
| Stems and leaves (10 cm) | 2.50 | ND |
| Stems (1 to 5 cm parts above ground) | 0.75 | ND |
| Roots (13 cm) | 2.50 | 1.8 × 103 ± 2.0 × 102 |
| Soil (rhizosphere) | 2.00 | 2.7 × 101 ± 8.5 × 100 |
| Plant 2 | ||
| Stems and leaves (10 cm) | 2.50 | ND |
| Stems (1 to 5 cm parts above ground) | 0.80 | ND |
| Roots (13 cm) | 3.10 | 1.9 × 104 ± 2.5 × 103 |
| Soil (rhizosphere) | 2.00 | 5.5 × 102 ± 1.5 × 102 |
| Plant 3 | ||
| Roots | 0.51 | 5.7 × 105 ± 3.0 × 104 |
| Soil (rhizosphere) | 2.00 | 7.1 × 105 ± 1.8 × 105 |
a
Plant 1, a control plant treated with φRSL1 only (in vitro treated and transferred to soil); plant 2, a plant treated with φRSL1, followed by the pathogen (in vitro treated and transferred to soil); plant 3, a φRSL1-treated plant with no wilting symptom 18 days after the pathogen challenge (in soil experiments).
b
Assays were repeated three times. ND, not detected.