Table 1.
Results of Probit analysis of the dose-response data (dose versus rate of detection) for Cryptosporidium nested PCR using plasmid templates of different species or genomic DNA templates of C. parvum and C. andersoni
| Species or genotype | Probit = 95a | 95% CL | Probit = 50 | 95% CL | χ2 (df, P)b |
|---|---|---|---|---|---|
| Plasmid DNA | |||||
| C. andersoni | 10.4 | 8.2–15.1 | 3.9 | 2.8–5.2 | 8.1 (10, 0.62) |
| C. parvum | 11.0 | 8.6–16.2 | 3.5 | 2.4–4.8 | 16.4 (14, 0.289) |
| C. muris | 11.3 | 8.2–20.4 | 4.3 | 2.7–6.7 | 20.8 (12, 0.054) |
| C. felis | 7.5 | 5.7–12.3 | 3.5 | 2.6–5.1 | 20.3 (14, 0.122) |
| C. ubiquitum | 8.0 | 6.0–13.2 | 3.0 | 1.9–4.4 | 20.2 (14, 0.124) |
| C. hominis | 8.5 | 6.6–12.6 | 2.6 | 1.6–3.6 | 18.1 (14, 0.201) |
| C. meleagridis | 8.8 | 7.2–12.0 | 4.0 | 3.2–5.1 | 18.2 (16, 0.310) |
| Genomic DNA | |||||
| C. andersoni | 7.3 | 4.1–9.1 | 2.5 | −8.3–4.8 | 81.8 (67, 0.106) |
| C. parvum | 10.4 | 8.6–13.0 | 3.7 | 0.2–5.7 | 95.1 (76, 0.068) |
a
Probit = 95 equals the LoD.
b
Model fit was assessed as a part of the Probit analysis and was determined by the Pearson goodness-of-fit chi-square statistic (a P value of >0.05 indicates an adequate model fit).