Table 2.

Detection of C. parvum or C. hominis in the presence of C. andersoni or C. ubiquitum as determined by nested PCR-RFLP for reaction mixtures containing a mixture of plasmid templates^b

^aTemplate copy numbers that are near or below the detection limit of the assay, indicating there is a high degree of variability in the number of positive replicates obtained at these copy numbers even in single-template reactions.

^bShading indicates plasmid template concentrations where a reduction or total loss of detection by nested PCR-RFLP occurred. Results in boldface type indicate results where plasmid templates were mixed at equal concentrations. The value below a diagonal line represents the number of nested PCR-RFLP mixtures positive for those species listed on the horizontal row; the value above the diagonal line is the number of nested PCR-RFLP mixtures positive for the species listed on the vertical column. NT, these ratios were not tested.