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. 2011 Jun;77(12):3998–4007. doi: 10.1128/AEM.02706-10

Table 4.

Distribution of species obtained from direct sequencing of limiting-template nested PCRs of whole-oocyst DNA extracts

No. of copies No. of sequences detecteda
C. parvum C. andersoni No sequence
6 of C. parvum and 6 of C. andersoni 3 14 2
6 of C. parvum and 30 of C. andersoni 2 9 4
30 of C. parvum and 6 of C. andersoni 13 5 6
a

Number of sequences detected from individual species by sequencing the cloned nested-PCR products from mixed, whole-oocyte DNA extracts.