Table 2.
Selected biomarkers for serovar-level identification of single serovars or groups of serovars (major serovar-identifying peaks)
| Exptl mass (avg m/z) | Tentative protein identity | Predicted mass (Da) | Serovar(s) in which biomarker found | Antigenic formula | Mass in other serovars (avg m/z) | Predicted mass (Da) | Amino acid exchange |
|---|---|---|---|---|---|---|---|
| 5,966 | Probably putative uncharacterized protein | NAa | Newport-I | 6,8:e,h:1,2 | 6,009 | 6,009.3b | NA |
| 6,022 | Putative uncharacterized protein YciG | 6,022.3a | Choleraesuis | 6,7:c:1,5 | 6,094 | 6,094.4 | E→G |
| 6,036 | Putative uncharacterized protein | 6,036.3b | Enteritidis | 1,9,12:g,m:- | 6,009 | 6,009.3b | S→N |
| 6,512 | Gns | 6,511.6 | Newport-I | 6,8:e,h:1,2 | 6,484 | 6,483.5 | A→V |
| Infantis | 6,7,14:r:1,5 | ||||||
| Manhattan | 6,8:d:1,5 | ||||||
| Mbandaka | 6,7,14:z10:e,n,z15 | ||||||
| 4,12:d:- | 4,12:d:- | ||||||
| Paratyphi A | 1,2,12:a:- | ||||||
| Stanleyville | 1,4,12:z4, z23:(1,2) | ||||||
| Zanzibar | 3,(10),(15):k:1,5 | ||||||
| Lexington | 3,10:z10:1,5 | ||||||
| 6,587 | Ribosome modulation factor | 6,586.6 | Paratyphi A | 1,2,12:a:- | 6,572 | 6,572.5 | D→E |
| 7,097 | Putative uncharacterized protein YaiA | 7,096.8b | Typhimurium/4,5,12:i:- | 4,5,12:i:(1,2) | 7,111 | 7,110.9b | K→N |
| Virchow | 6,7,14:r:1,2 | ||||||
| Newport-II | 6,8:e,h:1,2 | ||||||
| 7,139 | Putative uncharacterized protein YaiA | 7,138.9b | Heidelberg | 1,4,[5],12:r:1,2 | 7,111 | 7,110.9b | A→V |
| 7,983 | Uncharacterized protein YibT | NA | Derby-I | 1,4,[5],12:f,g:[1,2] | 7,993 | 7,993.1 | NA |
| 8,023 | Uncharacterized protein YibT | 8,023.1b | Heidelberg | 1,4,[5],12:r:1,2 | 7,993 | 7,993.1 | G→S |
| NA | Thompson | 6,7,14:k:1,5 | NA | ||||
| Blockley | 6,8:k:1,5 | ||||||
| Haardt | 8:k:1,5 | ||||||
| 8,027 | Uncharacterized protein YibT | 8,027.1a | Tennessee | 6,7,14:z29:[1,2,7] | 7,993 | 7,993.1 | L→F |
| 8,061 | Probably putative cytoplasmic protein YecF | NA | 4,12:d:- | 4,12:d:- | 8,051 | 8,051.2b | NA |
| 8,151 | RNA binding translation initiation factor IF-1 | 8,151.5b | Paratyphi A | 1,2,12:a:- | 8,119 | 8,119.4 | V→M |
| 8,342 | Putative stress response protein YjbJ | 8,342.3b | Tennessee | 6,7,14:z29:[1,2,7] | 8,329 | 8,329.3b | T→N |
| 9,548 | Probably DNA-binding protein HU-α | NA | Manhattan | 6,8:d:1,5 | 9,523 | 9,521.9 | NA |
| 10,048 | Ribosomal protein S15 | 10,048.5b | Virchow | 6,7,14:r:1,2 | 10,067 | 10,067.5b | R→H |
| 10,381 | Probably ribosomal protein L17 | NA | Schleissheim | 4,12,27:b:- | 14,395 | 14,395.6 | NAc |
| Gallinarum var. Pullorum | 1,9,12:-:- | ||||||
| 10,528 | Ribosomal protein L25 | 10,528.2 | Manhattan | 6,8:d:1,5 | 10,542 | 10,542.2 | I→V |
| Braenderup | 6,7:e,h:enz15 | ||||||
| Newport-III | 6,8:e,h:1,2 | ||||||
| Haifa | 1,4,[5],12:z10:1,2 | ||||||
| Stanley | 1,4,[5],12:z10:1,2 | ||||||
| 4,5,12,27:d:1,2 | |||||||
| 10,569 | Probably RL25 | NA | Schleissheim | 4,12,27:b:- | 10,542 | 10,542.2 | NA |
| 10,872 | Probable σ54 modulation protein | 10,872.4 | Heidelberg | 1,4,[5],12:r:1,2 | 10,858 | 10,858.4 | V→I |
| 10,886 | Probable σ54 modulation protein | 10,886.4 | Cholerasuis | 6,7:c:1,5 | 10,957 | 10,958.5c | K→R |
| 10,899 | Uncharacterized protein YbgS | 10,900.5c | Manhattan | 6,8:d:1,5 | 10,957 | 10,958.5c | D→G |
| Choleraesuis | 6,7:c:1,5 | ||||||
| Muenchen | 6,8:d:1,2 | ||||||
| Haifa | 1,4,[5],12:z10:1,2 | ||||||
| 10,927 | Uncharacterized protein YbgS | 10,928.5c | Hadar | 6,8:z10:e,n,x | 10,957 | 10,958.5c | T→A |
| Kottbus | 6,8:e,h:1,5 | ||||||
| Haardt | 8:k:1,5 | ||||||
| Blockley | 6,8:k:1,5 | ||||||
| Ferruch | 8:e,h:1,5 | ||||||
| Kiambu | 1,4,12:z:1,5 | ||||||
| 4,12:d:- | 4,12:d:- | ||||||
| Orion | 3,10:y:1,5 | ||||||
| Ealing-I | 35:g,m,s:- | ||||||
| 10,947 | Probably uncharacterized protein YbgS | NA | Brandenburg | 4,[5],12:l, v:enz15 | 10,957 | 10,958.5c | NA |
| Panama | 1,9,12:l,v:1,5 | ||||||
| Israel | 9,12:e,h:enz15 | ||||||
| Reading | 4,12:e,h:1,5 | ||||||
| Sandiego | 1,4,[5],12:e,h:enz15 | ||||||
| Eastbourne | 1,9,12:e,h:1,5 | ||||||
| 10,969 | Probably uncharacterized protein YbgS | NA | Aba | 6,8:i:enz15 | 10,957 | 10,958.5c | NA |
| Sandiego | 1,4,[5],12:e,h:enz15 | ||||||
| 10,988 | Uncharacterized protein YbgS | 10,988.5c | Heidelberg | 1,4,[5],12:r:1,2 | 10,957 | 10,958.5c | G→S |
| 11,591 | Ribosomal protein L21 | 11,591.8 | Paratyphi A | 1,2,12:a:- | 11,579 | 11,578.4 | V→I |
| 14,381 | Ribosomal protein RL17 | NA | Schleissheim | 4,12,27:b:- | NA | 14,395.6 | NA |
| 14,981 | Ribosomal protein RL15 | NA | London | 3,(10)(15):l,v:1,6 | 14,967 | 14,967.4 | NA |
| 15,342 | Probably DNA binding protein H-NS | NA | Paratyphi B-I | 1,4,[5],12:b:1,2 | 15,412 | 15,412.5b | NA |
| 15,471 | DNA binding protein H-NS | 15,470.5b | Paratyphi A | 1,2,12:a:- | 15,412 | 15,412.5b | G→D |
| 17,432 | Ribosomal protein RS7 | 17,432.1b | Choleraesuis | 6,7:c:1,5 | 17,461/474 | V→A | |
| 18,635 | Putative uncharacterized protein YciF | 18,634.2 | Infantis | 6,7,14:r:1,5 | 18,644/655 | 18,643.7 | R→H/P→S |
| Augustenborg | 6,7,14:i:1,2 | ||||||
| Colindale | 6,7:r:1,7 |
a
NA, no sequence data available.
b
Methionine loss.
c
Signal peptide cleavage.