Table 1.
Primer and probe sequences for the detection of EV and HuNoV
| Virus | Primer or probe | Sequence (5′–3′)a | Reference |
|---|---|---|---|
| Enterovirus (poliovirus) | Ev2 (forward) | CCCCTGAATGCGGCTAATC | 18 |
| Ev1 (reverse) | GATTGTCACCATAAGCAGC | ||
| Ev-probe | FAM-TGGGAGGGCGATCGCAATCT-TAMRA | ||
| Human norovirus genogroup I | COG1-F | CGYTGGATGCGNTTYCATGA | 11 |
| COG1-R | CTTAGACGCCATCATCATTYAC | ||
| RING1(a)-TP | FAM-AGATYGCGATCYCCTGTCCA-TAMRA | ||
| RING1(b)-TP | FAM-AGATCGCGGTCTCCTGTCCA-TAMRA | ||
| Human norovirus genogroup II | COG2-F | CARGARBCNATGTTYAGRTGGATGAG | 11 |
| COG2-R | TCGACGCCATCTTCATTCACA | ||
| RING2-TP | FAM-TGGGAGGGCGATCGCAATCT-TAMRA |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.