Fig. 1.

Plasmid pFW213. (A) Agarose gel electrophoresis of cesium chloride gradient-purified pFW213. SC, supercoiled DNA ladder. (B) Physical and genetic map of pFW213. Relative sizes, directions of transcription, and assigned designations are indicated. The assigned first base and positions of restriction sites used in subcloning pFW213 are listed in parentheses. (C) RT-PCR analysis of ORFs in pFW213. Two percent of the total cDNA generated from RT was amplified with specific primers, and 10% of the PCR products was run on a 1.8% TAE gel. C, products generated from total cellular DNA; +, reverse transcriptase was included in the reaction mixture; −, control groups without reverse transcriptase. A 1-kb DNA ladder was used as the marker.