Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Phenotypea | Description | Source or reference |
|---|---|---|---|
| Streptococcal strains | |||
| S. parasanguinis FW213 | Wild-type strain harboring pFW213 | 7 | |
| S. gordonii CH1 | Plasmid-free, naturally competent streptococcal host | D. J. LeBlanc | |
| S. mutans GS5 | Plasmid-free, naturally competent streptococcal host | H. K. Kuramitsu | |
| S. sanguinis SK36 | Plasmid-free, naturally competent streptococcal host | T. Kitten | |
| E. coli plasmids | |||
| pCTL2 | Kmr | pDL290 harboring an internal fragment of pFW213 (nt 2996 to 5589) at SacI and NsiI sites | This study |
| pCTL4 | Kmr | pDL290 harboring an internal fragment of pFW213 (nt 1064 to 3621) at EcoRI site | This study |
| pDL290 | Kmr | pSC101 replicon-based E. coli vector | D. J. LeBlanc |
| pSU21 | Cmr | p15A replicon-based E. coli vector | 3 |
| Streptococcal plasmids | |||
| pCG1 | Spr | Streptococcus-E. coli shuttle vector composed of p15A origin, pFW213 basic replicon, and a spe encoding Sp resistance in both E. coli and streptococci | This study |
| pCTL14 | Spr | pFW213::spe at Klenow fragment-treated ClaI site | This study |
| pCTLr2 | Spr | Derivative of pCTL14, containing pFW213 nt 3800 to 5129 | This study |
| pCTLr3 | Spr | Derivative of pCTL14, containing pFW213 nt 3800 to 6180 | This study |
| pCTLr4 | Spr | Derivative of pCTL14, containing pFW213 nt 3800 to 6897 | This study |
| pCTLr5 | Spr | Derivative of pCTL14, containing pFW213 nt 3800 to 500 | This study |
| pDL276 | Kmr | pVA380-1 replicon-based Streptococcus-E. coli shuttle vector | 11 |
| pFW213 | Cryptic | Naturally occurring plasmid in S. parasanguinis FW213 | This study |
| pT12 | Spr | ExoIII deletion derivative of pCTL14, containing pFW213 nt 900 to 5700 | This study |
| pT16R | Spr | ExoIII deletion derivative of pCTL14, containing pFW213 nt 3800 to 900 | This study |
a
Cm, chloramphenicol; Km, kanamycin; Sp, spectinomycin; r, resistant.