Table 2.

Primers used in this study

Primer	Nucleotide sequencea	Restriction site
yeaS-F	5′-CGGGATCCCGACGTCAGCGAAAGTGC-3′	BamHI
yeaS-R	5′-CGGGATCCCAGAAAGGCGTTGAGCG-3′	BamHI
yddG-F	5′-CGGGATCCAGTGCTTGCTTCGGGG-3′	BamHI
yddG-R	5′-CGGGATCCATAGACTTCGGCAGCGTG-3′	BamHI
ytfF-F	5′-CGGGATCCCGGCACTGAAAAGCGTCG-3′	BamHI
ytfF-R	5′-CGGGATCCAGAGAGCGCCAGTTCACC-3′	BamHI
ygaW-F	5′-CGGGATCCCGTTACCTCACCCCCAAAC-3′	BamHI
ygaW-R	5′-CGGGATCCCGCGAATGGGACGTACCG-3′	BamHI
ygaW15b-F	5′-CGGGATCCATATGTTCTCACCGCAGTCACG-3′	NdeI
ygaW15b-R	5′-CGGGATCCTCAGGCTTTTACCTGCTGGT-3′	BamHI
ΔytfF-F	5′-CGGATATCGATGATGTGGGCAACAGC-3′	EcoRV
ΔytfF-R	5′-CGGATATCTCATCGTGGAAACAGGCG-3′	EcoRV
BSalaD-F	5′-GATGTTTAGATAAATGAAGATTGGTATTCC-3′
BSalaD-R	5′-AAACTGCAGCTAATCCACCATAAATG-3′	PstI
SBPldh-F	5′-GCTGGATCCATAAATTGATGAATCAC-3′	BamHI
SBPldh-R	5′-GGAATACCAATCTTCATTTATCTAAACATC-3′
yeaSRT-F	5′-CGGTTATCTTGCGGCCTG-3′
yeaSRT-R	5′-TTGCAGCGTCGCCAGTCG-3′
yddGRT-F	5′-AGGCGGTGACAATGGGTTAC-3′
yddGRT-R	5′-TTTAATCATGACGGGCGTGC-3′
ytfFRT-F	5′-GCAGGGTTGATGTGGGGG-3′
ytfFRT-R	5′-GAATGACCACCGGCAGGG-3′
ygaWRT-F	5′-TTCTCACCGCAGTCACGC-3′
ygaWRT-R	5′-CTGCTGGTAACGGCTGAC-3′
gapART-F	5′-TGAATGGCAAACTGACTGGTATGGC-3′
gapART-R	5′-AACCGGTTTCGTTGTCGTACCAGGA-3′

^aUnderlines represent restriction sites in the tag sequences added.