Fig. 1.
Scheme of the strategy followed to engineer Lactococcus lactis for the synthesis of trehalose. PTS, phosphotransferase system; TrePP, trehalose 6-phosphate phosphorylase; β-PGM, β-phosphoglucomutase; TPPPf, trehalose 6-phosphate phosphatase from Propionibacterium freudenreichii; Tre6P, trehalose 6-phosphate; Glc6P, glucose 6-phosphate; β-Glc1P, β-glucose 1-phosphate; Glc, glucose; Tre, trehalose; Lac, lactate. The genes encoding TrePP, β-PGM, and TPPPf were overexpressed in L. lactis NZ9000 with the purpose of channeling Glc6P for the synthesis of trehalose.