Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| MG1363 | Plasmid-free derivative of SH4109 | 22 |
| NZ9000 | MG1363; pepN::nisR nisK | 32 |
| NZ9000(pNZ8020) | Derivative of NZ9000 carrying pNZ8020 | This work |
| NZ9000ΔccpA | NZ9000 chromosomal deletion of ccpA | This work |
| NZ9000ΔccpA(pNZotsB) | Derivative of NZ9000ΔccpA carrying pNZotsB | This work |
| NZ9000ΔccpA(pNZtpo) | Derivative of NZ9000ΔccpA carrying pNZtpo | This work |
| NZ9000(pNZtpo) | Derivative of NZ9000 carrying pNZtpo | This work |
| NZ9000(pNZotsBA) | Derivative of NZ9000 carrying pNZotsBA | This work |
| Propionibacterium freudenreichii subsp. shermanii NIZO B365 | 9 | |
| E. coli DH5α | Amersham Biosciences | |
| Plasmids | ||
| pORI280::ΔccpA | pORI280 derivative carrying ccpA up- and downstream regions | 65 |
| pNZ8020 | Cmr; nisin-inducible PnisA | 13 |
| pNZotsB | pNZ8020 with P. freudenreichiiotsB cloned into the BamHI/EcoRI site; Cmr | This work |
| pNZtrePPpgmB | pNZ8020 with the lactococcal trePP and pgmB cloned into the SpeI/SacI site; Cmr | This work |
| pNZtpo | pNZtrePPpgmB with P. freudenreichiiotsB cloned into the SacI/XbaI site; Cmr | This work |
| pNZotsBA | pNZ8048 with E. coliotsB and otsA cloned into the NcoI/XbaI site; Cmr | This work |
a
Cmr, resistance to chloramphenicol.