Table 2.

Trehalose contents in ethanol extracts (intracellular trehalose) or in the growth medium (extracellular trehalose) of L. lactis cells collected during mid-exponential and stationary phases of growth^a

Strain	Mean extracellular trehalose concn (mM) ± SD		Mean intracellular trehalose concn (mM) ± SD		Mean total trehalose (μmol/mg protein)
	Exp	Stat	Exp	Stat	Exp	Stat
NZ9000ΔccpA(pNZtpo)	1.2b	0.4b	170.8b	6.2b	3.9b	0.4b
NZ9000(pNZtpo)	0.8 ± 0.1	2.1 ± 0.1	167.0 ± 9.2	123.1 ± 7.4	2.2 ± 0.2	2.6 ± 0.3
NZ9000(pNZotsBA)	0.4 ± 0.1	1.2 ± 0.04	79.2 ± 18.1	75.7 ± 1.3	1.2 ± 0.3	1.4 ± 0.04

^aCultures were grown with the pH controlled at 6.5. Trehalose was quantified by proton NMR. Exp, mid-exponential phase; Stat, stationary phase.

^bA single experiment was performed.