Fig. 2.
Gas chromatograms of various lipid extracts of G. aggregans TPB6028T. Shown are the fatty acids released after acid (a) and alkaline (b) hydrolyses of whole-cell material and the fatty acids present in the Bligh-Dyer extract (c), the acid-hydrolyzed Bligh-Dyer extract (d), the alkali-hydrolyzed Bligh-Dyer extract (e), and the alkali-hydrolyzed residue after Bligh-Dyer extraction (f). Fatty acids were derivatized to the corresponding methyl esters prior to GC analysis. Key: 2, iso-diabolic acid; 6, iso-C15 fatty acid; 7, C16:1ω7c fatty acid; 8, C16:0 fatty acid; 9, iso-C17 fatty acid. An asterisk denotes a plasticizer contaminant (phthalate). Note the abundance of iso-diabolic acid in the hydrolysates from the cell material and residue after extraction and its absence from the solvent extract.