Table 2.
Fatty acid composition after acid hydrolysis of cell material and presence of branched GDGTs in the acidobacterial strains studied
| Component | % of total fatty acidsa in strainb: |
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1c | 2c | 3c | 4d | 5c | 6c | 6d | 7d | 8d | 9d | 10d | 11d | 12c | 13d | 14c | 15c | 16d | 17d | |
| iso-C13 | 0.2 | 0.2 | 5.4 | 5.2 | 2.3 | |||||||||||||
| iso-C14 | 2.0 | 1.6 | ||||||||||||||||
| C14:1ω5 | 0.1 | 1.7 | 0.1 | 0.4e | 0.2 | 0.4 | 0.1 | 0.5 | 0.6 | 0.1 | 0.3 | |||||||
| C14:0 | 0.5 | 0.5 | 0.5 | 1.8 | 0.8 | 0.9 | 0.5 | 1.5 | 1.9 | 1.1 | 1.0 | 1.2 | 1.7 | |||||
| iso-C15:1ω6 | 0.4 | 3.8f | 0.2 | 0.1 | 0.2 | 0.1 | ||||||||||||
| iso-C15 | 38.7 | 34.2 | 29.2 | 46.0 | 32.1 | 36.1 | 38.8 | 35.0 | 37.0 | 30.0 | 33.0 | 32.5 | 35.1 | 27.0 | 22.4 | 26.0 | 16.0 | 12.0 |
| anteiso-C15 | 7.3 | 0.2 | 0.1 | 1.1 | 0.8 | 0.5 | 0.8 | |||||||||||
| C15:1ω6 | 0.1 | 0.8 | 0.3 | 4.4g | 0.4 | 0.1 | 0.3 | 0.5 | ||||||||||
| C15:0 | 0.2 | 1.4 | 0.2 | 0.2 | 0.6 | 0.4 | ||||||||||||
| iso-C16 | 0.8 | 2.6 | 0.6 | |||||||||||||||
| C16:1ω7c | 0.2 | 0.3 | 9.9 | 2.0 | 3.2 | 26.0 | 21.0 | 22.0 | 8.4 | 18.5 | 20.3 | 18.0 | 21.5 | 23.7 | 23.0 | 32.0 | ||
| C16:1ω7t | 0.4 | 0.3 | 1.2 | 0.4 | 0.2 | 0.3 | 2.1 | 0.8 | 0.4 | |||||||||
| C16:1ω6+ω5 | 0.3 | 1.6 | 0.1 | |||||||||||||||
| C16:0 | 3.0 | 0.6 | 4.8 | 5.8 | 3.2 | 7.0 | 5.0 | 11.0 | 9.8 | 6.0 | 6.8 | 7.3 | 7.6 | 5.6 | 15.0 | 18.0 | ||
| iso-C17:1ω8c | 19.5 | 14.6 | 17.4 | 18.4 | 1.0 | 0.5 | 0.8 | 0.8 | 0.2 | 0.6 | 0.1 | 0.2 | 0.4 | 0.3 | 1.1 | 0.3 | ||
| iso-C17:1ω8t | 0.9 | 1.3 | 0.5 | 1.5 | ||||||||||||||
| iso-C17 | 5.8 | 6.1 | 8.5 | 6.8 | 1.0 | 1.7 | 1.5 | 1.7 | 1.6 | 3.0 | 2.2 | 1.7 | 0.5 | 0.7 | 0.7 | 2.0 | 2.7 | 1.0 |
| anteiso-C17 | 0.4 | 0.9 | 4.1 | 1.8 | 0.2 | 0.6 | 0.6 | 1.0 | 1.0 | 0.7 | 2.4 | 0.5 | ||||||
| C17:1ω8 | 0.3 | 1.4 | 0.9 | 0.2h | 0.1h | 0.1 | ||||||||||||
| C17:0 | 0.8 | 0.2 | 1.3 | 0.3 | 0.1 | 0.5 | 0.4 | 0.6 | 0.7 | 0.3 | 0.5 | 0.4 | ||||||
| C18:1ω9 | 3.4 | 11.1 | 15.4 | 0.4 | 2.0 | |||||||||||||
| C18:1ω7 | 2.9 | 0.7 | 0.1 | 0.1 | 3.8 | 0.2 | 0.9 | 0.4 | ||||||||||
| C18:0 | 2.6 | 0.6 | 4.0 | 1.8 | 0.4 | 0.4 | 1.0 | 0.5 | 0.5 | 1.4 | 0.8 | 1.5 | 1.2 | |||||
| iso-C19 | 2.7 | |||||||||||||||||
| iso-Diabolic acidi | 34.7 | 29.9 | 30.3 | 21.5 | 39.4 | 35.6 | 33.4 | 26.0 | 28.0 | 29.0 | 29.0 | 31.5 | 33.1 | 36.0 | 42.8 | 36.1 | 33.0 | 29.0 |
| iso-C15-monoglycerol ether | 0.2 | 0.5 | ||||||||||||||||
| C30 glycerol etherj | 3.2 | 3.5 | ||||||||||||||||
| GDGTk | − | ± | − | − | √ | − | − | − | − | − | − | − | − | − | − | √ | − | − |
Values for major components (i.e., >3% of the total fatty acids) are underlined.
Strains: 1, Acidobacteriaceae strain 277; 2, Acidobacteriaceae strain 307; 3, Acidobacteriaceae strain A2-1c; 4, Acidobacteriaceae strain KA1; 5, Acidobacteriaceae strain A2-4c; 6, A. capsulatum 161T; 7, B. elongata SN10T; 8, G. pectinivorans TPB6011T; 9, G. aggregans TPB6028T; 10, G. paludicola LCBR1; 11, G. paludicola OB1010T; 12, T. roseus KBS63T; 13, Terriglobus sp. strain KMR; 14, E. modestus Jbg-1T; 15, E. aggregans Wbg-1T; 16, B. aggregatus MPL3T; 17, B. aggregatus MPL1011T.
Grown at DSMZ.
Grown at the Winogradsky Institute of Microbiology.
ω7 instead of ω5.
ω8 instead of ω6.
ω7 instead of ω6.
ω6 instead of ω8.
Structure 2 in Fig. 1.
Structure 3 in Fig. 1.
Structure 1 in Fig. 1. −, not detected; ±, detected by single-ion monitoring MS but not confirmed by full mass spectrum; √, present, confirmed by full mass spectrum.