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. 2010 Jan 11;2:1–11. doi: 10.2147/HMER.S7673

Figure 3.

Figure 3

LPS activates stress activated kinases in hepatocytes. Cultured rat hepatocytes were treated with 500 ng/mL LPS for the time points indicated and cell lysates were prepared. The lysates were immunoblotted for phospho JNK (A and D), phospho p38 MAPK (B and D), and phospho AKT (C and D). The blots were developed by chemiluminescence, digitized and quantified. Results of quantification of at least 3 separate experiments are shown in A B and C and representative gels in D. Hepatocytes were pretreated for 30 min with either a JNK (SP 600125, 15 μM) or p38 MAPK inhibitor (SB 203580, 5 μM) and then overnight with 500 ng/mL of LPS (E). Cells were evaluated morphologically by Hoechst staining for the presence of apoptosis. Results are expressed as the percentage increase in apoptosis compared to control and represent mean ± SD (n = 3). *Significantly different from control, #Significantly different from LPS, P < 0.05.

Abbreviations: LPS, lipopolysaccharide; JNK, c-Jun N-terminal kinase; pJNK, phospho JNK; MAPK, p38 mitogen-activated protein kinases; AKT, protein kinase; SD, standard deviation.