Figure 4.

Wingless HSF binds DNA with less affinity than wild-type. (A) Gel shift assay with wingless (lanes 1–8) and wild-type (lanes 9–16) HSF fragments binding to a 3-box template. Samples contained increasing amounts of protein going from right to left, with the highest protein amount of 2 µM (lanes 1 and 9) and undergoing 3-fold dilutions for each subsequent lane. Lane 17 contained only labeled DNA substrate. The binding curves from the quantitation of gel shift assays using wild-type (B) or wingless (C) HSF are shown. Three separate experiments were quantitated as described in Materials and Methods. The apparent K_D value is given for each plot.