Table 1. DNA substrates used in the gel shift studies.
Substrate |
Sequence (5′–3′) |
3-box |
gccaGAAcaTTCcaGAAcacc |
4-box HH |
gccaTTCcaGAAcaTTCcaGAAcacc |
4-box TT |
gccaGAAcaTTCcaGAAcaTCCcacc |
2-box HH |
gatgccGAAccTTCcatgcc |
2-box TT | CatgccTTCccGAAcatgcc |
One strand of the duplex substrates is shown. Opposite strands were complementary and formed blunt-ended substrates. GAA binding sites are capitalized.