. Author manuscript; available in PMC: 2012 Jun 1.

Published in final edited form as: J Bioenerg Biomembr. 2011 Apr 20;43(3):227–236. doi: 10.1007/s10863-011-9353-8

Table 3.

Marker enzymes for various organelles of 6 month old control and CR mice

Enzymes	M1	M3	M10	Cytosol
CS
Control	0.706±0.020	0.332±0.016	0.064±0.008	0.008±0.0003
CR	0.610±0.025^*	0.290±0.009^*	0.049±0.006	0.008±0.0002
LDH
Control	0.063±0.010	0.129±0.024	0.216±0.041	4.202±0.318
CR	0.058±0.007	0.190±0.037	0.172±0.015	3.126±0.253^*
AP
Control	0.164±0.023^a	0.265±0.018^b	1.099±0.066^c	–
CR	0.178±0.020^a	0.252±0.021^b	0.990±0.059^c	–
UO
Control	0.026±0.004^a	0.030±0.004^a	0.304±0.029^b	–
CR	0.022±0.006^a	0.033±0.005^a	0.299±0.026^b	–
G6Pase
Control	0.010±0.003^a	0.013±0.001^a	0.124±0.009^b	–
CR	0.011±0.002^a	0.013±0.001^a	0.133±0.006^a	–

CS citrate synthase, LDH lactate dehydrogenase, AP acid phosphatase, UO urate oxidase, G6Pase glucose-6-phosphatase

Values represent mean±SEM for n=6 animals per group

P<0.05 for control versus CR within a mitochondrial or cytosolic fraction. Values within a row which do not share a common letter differ significantly (P<0.05)