Table 2. Effects of the Most Potent PIB-SOs, CA-4, and Compound 6 on Cell Cycle Progression, Cytoskeleton Integrity, and Results of a Competition Assay with EBI.
For cell cycle progression, M21 cells were incubated in presence of PIB-SOs at 5 times their respective IC50 for 24 h.
For competition assay with EBI, MDA-MB-231 cells were incubated in the presence of PIB-SOs at 1000 times their respective IC50 for 2 h and afterward in the presence of 100 μM EBI for 1.5 h.
Inhibition coding: +++, strong inhibition; ++, significant inhibition; +, weak inhibition; −, no inhibition; N/A, not applicable.
M21 cells were treated for 16 h with the drug at 5 times their respective IC50, and cellular microtubule structures were visualized using indirect immunofluorescence using an anti-β-tubulin monoclonal antibody.



