Fig. 4.

Effect of CrVI on translocation of Bcl-2 family proteins and heat shock proteins 70, and 90 in the mitochondria and cytosol. Cells were pre-treated with or without vitamin C for 24 h and treated with CrVI (10 μM) for 0 h, 12 h and 24 h. Western blots were performed with 75 μg of protein, as described in Materials and methods. A, Representative western blots of Bcl-2, Bcl-XL, BAX, total Bad (t-Bad), phosphorylated Bad (pBad-112, pBad-136), HSP-70 and HSP-90 in the cytosolic and mitochondrial protein fractions. M- mitochondrial protein fraction; C-cytosolic protein fraction. B, Histograms of Integrated Density Value (IDV) for each protein. Each value is the mean±SEM of three experiments, P<0.05; a: CrVI-treatment, 0 h vs 12 h or 24 h; b: CrVI+Vitamin C-treatment, 0 h vs 12 h or 24 h. Vit.C—Vitamin C.