Blebbing is a universal cellular response to plasmalemmal injury. Blebbing was recorded in SLO-perforated primary cultures of human myometrial smooth muscle cells, Jurkat T cells and Raji B cells, in mechanically injured human astrocytoma cells (U 373 cells), and in HEK 293 cells that had been treated with bacterial phospholipase C. Raji cells are shown at low magnification: blebbing cells are marked by asterisks. The images of non-transfected (Raji and Jurkat) or the CFP-expressing cells (smooth muscle, U 373, HEK 293) are presented in grey-scale mode. Magnification bar=10 μm