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Intrableb elevation in Ca2+. SLO was added (time=0) to non-transfected HEK 293 cells that had been pre-loaded with Fluo 5F. At time points=90, 108 and 117 s, [Ca2+] was higher in the bleb (arrow) than in the cytoplasm. Fluorescence is shown in the rainbow mode: low [Ca2+] – blue; high [Ca2+] – red. Magnification bar=10 μm
