Figure 2.

hRFPL1 alters cell number increase over time. (a) Time course of HeLa cell number transduced with eGFP or hRFPL1. ^***P<0.001 compared with eGFP-expressing cells using two-way ANOVA followed by Tukey's post hoc test. (b) Identification of protein domains involved in hRFPL1 effect on cell number 3 days after transduction. Color legend: RFPL-specifying helix (RSH), orange; RING finger, blue; RFPL-defining motif (RDM), green; PRY, yellow; SPRY, red. The RING finger associated to the RDM form a predicted larger zinc finger of the RING/U-Box superfamily, and the presence of both PRY and SPRY form a B30.2 domain. ^***P<0.001 compared with eGFP-expressing cells using one-way ANOVA followed by Tukey's post hoc test