Table 1. hRFPL1 reduces the number of mitotic events.
Events and cell-cycle parameters | GFP-expressing cells (%) | hRFPL1-expressing cells (%) |
---|---|---|
3 mitotic divisions | 23.3±3.5 | 1.7±0.3 |
2 mitotic divisions | 65.5±6.7 | 18.0±2.0 |
1 mitotic division | 9.5±2.5 | 24.6±4.0 |
Incomplete mitosis | 0 | 13.1±1.2 |
No mitotic division | 0 | 13.1±2.7 |
Apoptosis | 1.7±0.5 | 29.5±3.5 |
The different parameters were quantified during 48 h using live-cell imaging experiments in GFP- or hRFPL1-expressing HeLa cells. Apoptosis was defined by typical morphological changes (cell shrinkage, blebbing).