Figure 6.

Human memory CD4⁺ T cells form a more efficient Fas DISC. (a) Immunoblot analysis of DISC components in naïve and memory human CD4⁺ T cells after stimulating (30 min) with anti-Fas antibody and immunoprecipitating with protein A/G beads as indicated. Lysates from both anti-Fas-treated and control cells contained low levels of p41/43 processed caspase-8, likely because of autoprocessing after lysis, T-cell activation or spontaneous cell death. Lines represent lanes not shown and the data are a representative of eight independent experiments. (b) Densitometry analysis of recruited DISC components normalized to Fas in DISC is shown. Right panel compares caspase-8 cleavage as a ratio of unprocessed versus cleaved caspase-8 in the DISC