Figure 7.

CARF suppression in vivo induces tumor regression. A549 cells were infected with MOI from 0.2–5 of shCARF-carrying adenovirus to determine the optimal dose (a). Nude mice (n=5–6 per group) were injected with 1 × 10⁷ A549 cells, and when tumors reached 100 mm³ in volume, either 3 × 10⁸ plaque forming unit of Ad-ΔB7 (squares) or Ad-ΔB7-shCARF (gray circles) were injected intratumorally three times every 2 days, at which time the tumor size was also measured (b). Survival of the animals was also recorded as percentage (%) viability (c). The mice were killed on day 50