Figure 3.

The fibronectin receptor confers capacity for Gal-1-stimulated anoikis to α₅-deficient colon cancer cells. (a) Cell surface binding of Gal-1 in mock- and α₅-transfected (clone α₅/1) Caco-2 cells. Number inserts indicate the amount of biotinylated Gal-1 added (μg/ ml). (b and c) Determination of Gal-1-stimulated anoikis after incubation with 125 μg/ml Gal-1 for 24 h (open bars) and α₅-expression levels (closed bars) in different clones of α₅-transfected Caco-2 cells (α₅/1 to α₅/4), wild-type (wt) cells and mock control (panel b) and in two α₅-transfected HT29 clones (α₅/1 and α₅/2), wt cells and mock control (panel c). Data represent at least three independent experiments (anoikis as fold stimulation without treatment, ^*P<0.05, ^**P<0.01, ^***P<0.001) and a representative determination of α₅ expression