Figure 1.

Classification of human glioma cell lines according to their sensitivity to THC-induced cell death. (a) Effect of THC on the viability (72 h) of 10 human glioma cells lines (cells with IC₅₀<2.5 μM (left panel) and cells with IC₅₀>2.5 μM (right panel) were considered sensitive and resistant, respectively, to THC-induced cell death; mean±S.D.; n=5). (b) CB₁ and CB₂ expression of the human glioma cells lines as determined by immunostaining (left panel) and western blot (right panel). One representative experiment of three is shown. (c) Effect of THC, SR1 (1 μM) and SR2 (1 μM) on the viability (72 h) of U87 (left panel) and T98 (right panel) cells (mean±S.D.; n=3; ^**P<0.01 from vehicle-treated cells, ^#P<0.05 and ^##P<0.01 from THC-treated cells; Veh, Vehicle, THC, Δ⁹-tetrahidrocannabinol)