Figure 4.

miR-885-5p directly targets CDK2 and MCM5. Predicted miR-885-5p target sites in the CDK2 and MCM5 3′-UTRs. Lines indicate perfect matches; colons, G:U pairs. Luciferase reporter assays of stably transfected cells and miRNA-mimic-transfected cells (indicated on x-axis) transiently transfected with either empty vector or vector containing wild-type or mutant miR-885-5p binding sites (indicated as bar color). Firefly luciferase values were normalized to renilla activity. The highest normalized luciferase activity of the stable transfected cells or cells transfected with miRNA mimics was set to 1, and values are reported as fold increase (mean relative luciferase activity±S.D.). ^*P=<0.1; ^**P=<0.05; ^***P=<0.01 versus vector with mutant miR-885-5p binding sites