Figure 5.

DICER1 silencing modulates the expression of the p-ΔNp63α-dependent miRNAs upregulated after CIS exposure. Wild-type ΔNp63α cells were transfected with scrambled siRNA (Scr) and DICER1 (DCR) siRNA for 24 h and exposed to Con (−, Con) or 10 μg/ml CIS (+, CIS) for an additional 24 h. (a) DICER1 expression was analyzed by immunoblotting with indicated antibodies. Borders between images indicate that the data were obtained from separate experiments. Loading levels were assessed with anti-β-actin antibody. (b) Cell survival assay. (c) FACS analysis of cells. (d) Expression of mature miR-630 and miR-885-3p. (e) Expression of mature miR-181a, miR-519a and miR-374a. miRNA levels were examined by qPCR assay using TaqMan analysis and the data were plotted as relative units (RU). Values obtained with Con (−, Con) were designated as 1, with fold changes presented as numerical values. Experiments were performed in triplicate with ±S.D. as indicated (<0.05)