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. 2011 Jan 28;18(7):1220–1230. doi: 10.1038/cdd.2010.188

Figure 7.

Figure 7

CIS-induced modulation of miR-519a expression leads to the activation of cell cycle arrest and apoptotic markers. Wild-type ΔNp63α cells were transfected with control (mock), mimic, or inhibitor for miR-519a for 24 h and then exposed to Con (−) or 10 μg/ml CIS (+) for an additional 24 h. (a) Protein levels of PARP1, CDKN1A, YES1, and TP73 were examined by immunoblotting with the indicated antibodies, and loading levels were tested with an anti-β-actin antibody. (b) CIS-induced modulation of cell survival by miR-519a. Wild-type ΔNp63α cells were transfected with control (mock, curves 1 and 2), miR-519a mimic (curve 3), or miR-519a inhibitor (curve 4) and then exposed to Con (curves 1, 3, and 4) or 10 μg/ml CIS (curve 2) for 0–120 h. Cell survival was assessed at 24, 48, 72, 96, and 120 h by MTT assay. Experiments were performed in triplicate with ±S.D. as indicated (<0.05)