Figure 9.

CIS-induced activation of miR-630 expression leads to the modulation of apoptotic markers. Wild-type ΔNp63α cells were transfected with control (mock), mimic, or inhibitor for miR-630 for 24 h and then exposed to Con (−) or 10 μg/ml CIS (+) for an additional 24 h. (a) Protein levels of BCL2, BCL2L2, and YAP1 were examined by immunoblotting with the indicated antibodies, and loading levels were tested with an anti-β-actin antibody. (b) CIS-induced modulation of cell survival by miR-630. Wild-type ΔNp63α cells were transfected with control (mock, curves 1 and 2), miR-630 inhibitor (curve 3),³⁷ or miR-630 mimic (curve 4) and then exposed to Con (curve 1, 3, and 4) or 10 μg/ml CIS (curve 2) for 0–120 h. Cell survival was assessed at 24, 48, 72, 96, and 120 h by MTT assay. Experiments were performed in triplicate with ±S.D. as indicated (<0.05)