Figure 3.

eel-1 mutants are not defective in DNA damage checkpoint function. (a) eel-1(ok1575) mutants undergo a transient arrest in proliferation of mitotic germ cells in response to IR that is indistinguishable from wild-type or cep-1(gk138) mutants. Germlines are outlined in solid black lines and arrows indicate normal mitotic nuclei. Dotted arrows indicate enlarged mitotic nuclei 20 h after exposure to 120 Gy of IR. (b) Quantification of the number of nuclei per unit area in the mitotic region of the germline as shown in panel a. Data represent mean±S.E.M. of two independent experiments. Between 10 and 15 germlines were scored per experiment. (c) Sensitivity of L1 stage eel-1 mutants to IR-induced sterility is similar to wild type. Twenty animals of the indicated genotype were irradiated at the L1 stage and fertility assessed after they developed into adults