Figure 6.

eel-1 acts upstream or independently of ced-9. (a and b) Hermaphrodites of the indicated genotypes were irradiated with 60 Gy of IR at the L4 larval stage and germ cell apoptosis quantified 24 h later. Data represent mean±S.E.M. of three independent experiments. Between 15 and 30 germlines were scored per experiment. (c) Endogenous CED-9 protein levels are not affected by eel-1 status or IR. Hermaphrodites of the indicated genotype were treated with 120 Gy of IR at the L4 stage and the protein was extracted from whole-worm lysates after 24 h. Western analysis was performed using an anti-CED-9 antibody kindly provided by Dr. Barbara Conradt. The protein α tubulin was used as a loading control. Quantification was performed using ImageJ software. Protein abundance is relative to wild type at the indicated dose of IR