Table 2.
Salmonella Typhimurium Genes Involved in Motility That Are Regulated by luxS/Autoinducer-2 at Mid-Log Phase in the Absence of Glucose
| Genes | Putative identification of JCVI database | Fold change (W/M) | Fold change (wCFS/mCFS) |
|---|---|---|---|
| Chemotaxis proteins | |||
| Two-component system proteins | |||
| cheA | Chemotaxis protein CheA | −1.3 | 1.0 |
| cheB | Chemotaxis-specific methylesterase | 1.3 | −1.0 |
| STM2314 | Putative chemotaxis signal transduction protein | 3.5 | 1.1 |
| MCPs | |||
| tcp | Methyl-accepting transmembrane citrate/phenol chemoreceptor | 2.0 | −1.0 |
| STM3216 | Putative methyl-accepting chemotaxis protein | 1.8 | 1.1 |
| trg | Methyl-accepting chemotaxis protein III | 2.1 | 1.1 |
| aer | Aerotaxis sensor receptor | 2.5 | −1.1 |
| Flagellar assembly proteins | |||
| Type III secretion | |||
| fliH | Flagellar assembly protein H | −2.1 | −1.0 |
| fliO | Flagellar biosynthesis protein FliO | −1.8 | −1.0 |
| Motor/switch | |||
| motA | Flagellar motor protein MotA | 2.0 | −1.0 |
| motB | Flagellar motor protein MotB | 2.1 | −1.0 |
| C-ring | |||
| fliG | Flagellar motor switch protein G | −2.3 | −1.0 |
| fliN | Flagellar motor switch protein FliN | 1.2 | −1.0 |
| M, S, P, and L rings | |||
| flgA | Flagellar basal body P-ring biosynthesis protein FlgA | 1.5 | −1.0 |
| Rod, hook, and filament | |||
| flgB | Flagellar basal body rod protein FlgB | −2.4 | −1.1 |
| flgE | Flagellar hook protein FlgE | 1.3 | 1.1 |
| flgJ | Peptidoglycan hydrolase | −2.1 | −1.0 |
| flgK | Flagellar hook-associated protein FlgK | 2.2 | 1.1 |
| flgL | Flagellar hook-associated protein FlgL | 1.4 | 1.0 |
| fliD | Flagellar capping protein | 2.0 | 1.2 |
| fliK | Flagellar hook-length control protein | 1.2 | 1.0 |
| fljB | Flagellin | −1.3 | −1.0 |
| Regulation | |||
| flhC* | Regulator of flagellar biosynthesis, acts on class | 1.4 | −1.0 |
| flhD* | Regulator of flagellar biosynthesis, acts on class | 1.3 | −1.0 |
| flgM | Anti-sigma28 factor FlgM | 2.7 | −1.1 |
| Chaperones | |||
| fliJ | Flagellar biosynthesis chaperone | 1.5 | 1.1 |
| Pilus system | |||
| Fimbrial proteins | |||
| fimA | Fimbrin | 2.0 | −1.0 |
| fimD | Outer membrane usher protein precursor | −2.1 | 1.0 |
| Putative motility | |||
| lpfA | Long polar fimbrial protein A precursor | 1.4 | −1.1 |
| stdC | Putative fimbrial chaparone | 1.4 | −1.0 |
| cheM | Methyl-accepting chemotaxis protein II | 1.6 | 1.0 |
| stiA | Putative fimbrial subunit | 1.2 | −1.0 |
| lpfE | Long polar fimbrial minor protein | 1.2 | −2.2 |
| yifL | Putative outer membrane lipoprotein | 1.2 | −1.0 |
| stbA | Putative fimbrial major subunit | 1.2 | 1.1 |
Differentially regulated genes between wild type (w) and ΔluxS mutant (M) and between wild-type cell-free supernatants and ΔluxS mutant cell-free supernatants are listed. Genes that have the same direction of regulation in both W/M and wCFS/mCFS are regulated by AI-2 and are shown in bold.
wCFS, wild-type cell-free supernatants; mCFS, mutant CFS.