Table 4.

Salmonella Typhimurium Genes Involved in Virulence Regulated by luxS/Autoinducer-2 at Mid-Log Phase in the Absence of Glucose

Genes	Putative identification of JCVI database	Fold change W/M	Fold change wCFS/mCFS
hha	Hemolysin expression modulating protein (involved in environmental regulation)	1.9	1.0
pagP	PhoPQ-activated gene	−1.4	1.3
mviM	Putative virulence factor	2.2	1.3
cobB	Putative nicotinate-nucleotide dimethylbenzimidazolephosphoribosltransferase, homolog of virulence factor	1.6	1.2
pagC	PhoP regulated: reduced macrophage survival	1.5	−1.8
hnr	Response regulator in protein turnover: mouse virulence	−1.4	−1.3
sirC	Regulation of invasion genes	−2.3	−1.2
virK	Virulence gene homologous sequence to virK in	−2.5	1.4
avrA	Putative inner membrane protein	1.4	−3.1
STM2868	Putative cytoplasmic protein	−1.8	−2.1
orgA	Putative flagellar biosynthesis/type III secretory pathway protein	−1.7	−2.7
prgI	Cell invasion protein cytoplasmic	−1.4	−9.6
hilD	Regulatory helix-turn-helix proteins, araC family	1.2	−3.3
hilA	Invasion genes transcription activator	−2.0	−6.1
sptP	Protein tyrosine phosphate	−1.3	−5.9
sicP	Chaparone, related to virulence	−1.4	−5.0
STM2880	Putative cytoplasmic protein	−1.4	−2.9
sipB	Cell invasion protein	1.3	−2.8
spaR	Surface presentation of antigen secretory proteins	−1.5	−9.2
invC	Surface presentation of antigen secretory proteins	−1.5	−11.2
invB	Surface presentation of antigen secretory proteins	−1.4	−7.5
invA	Invasion protein	−1.6	−6.8
invE	Invasion protein	−1.4	−10.5
ygdP	Putative invasion protein NTP pyrophosphohydrolase	1.4	1.0
fis	Site-specific DNA inversion stimulation factor	−4.6	−1.0
hfq	Host factor I for bacteriophage Q beta	1.4	1.2

Differentially regulated genes between wild type (W) and ΔluxS mutant (M) and between wild-type cell-free supernatants and ΔluxS mutant cell-free supernatants are listed. Genes that have the same direction of regulation in both W/M and wCFS/mCFS are regulated by AI-2 and are shown in bold.