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Representative cross-sectional LSCM image of a film-coated microwell of a film-coated array after culturing HEK 293T/17 cells in the wells for three weeks. Cells in the topmost layer of the 3-D cell cluster were stained with Hoechst nuclear stain and WGA-594 membrane stain prior to imaging. The dotted white line indicates the surface of the microwell, which is 300 µm across and 120 µm deep, and is included to guide the eye.
