Table 3.
Vertebral microarchitecture assessed by μCT of 1, 3 and 6 month old male and female Grem1 null mice and wild type littermate controls.
| Males | 1 Month | 3 Month | 6 Month | |||
|---|---|---|---|---|---|---|
| Wild Type | Grem1 null | Wild Type | Grem1 null | Wild Type | Grem1 null | |
| Bone Volume/Tissue Volume (%) | 14.9 ± 0.9 | 11.8 ± 0.6* | 24.6 ± 0.7 | 16.9 ± 1.3* | 15.5 ± 1.5 | 10.1 ± 1.3* |
| Trabecular Separation (μm) | 196 ± 5 | 209 ± 5 | 183 ± 4 | 235 ± 10* | 224 ± 15 | 282 ± 13* |
| Trabecular Number (1/mm) | 5.0 ± 0.1 | 4.7 ± 0.1 | 5.6 ± 0.1 | 4.4 ± 0.2* | 4.5 ± 0.3 | 3.6 ± 0.1* |
| Trabecular Thickness (μm) | 35.1 ± 0.9 | 32.2 ± 0.2* | 45.4 ± 0.9 | 42.7 ± 0.7* | 37.7 ± 1.6 | 36.4 ± 1.8 |
| Connectivity Density (1/mm3) | 322 ± 23 | 264 ± 9 | 221 ± 15 | 159 ± 12* | 188 ± 17 | 113 ± 20* |
| Structure Model Index | 14.1± 0.1 | 1.6 ± 0.1 | 0.4 ± 0.1 | 0.9 ± 0.1* | 0.9 ± 0.1 | 1.4 ± 0.2* |
| Females | 1 Month | 3 Month | 6 Month | |||
| Wild Type | Grem1 null | Wild Type | Grem1 null | Wild Type | Grem1 null | |
| Bone Volume/Tissue Volume (%) | 16.6 ± 0.9 | 12.4 ± 0.9* | 20.6 ± 1.6 | 18.4 ± 2.7 | 15.1 ± 1.2 | 11.9 ± 2.0 |
| Trabecular Separation (μm) | 185 ± 8 | 213 ± 11 | 232 ± 10 | 256 ± 27 | 244 ± 8 | 306 ± 26 |
| Trabecular Number (1/mm) | 5.4 ± 0.2 | 4.7 ± 0.2* | 4.6 ± 0.2 | 4.3 ± 0.4 | 4.2 ± 0.1 | 3.5 ± 0.4 |
| Trabecular Thickness (μm) | 35.2 ± 1.2 | 32.7 ± 1.1 | 45.9 ± 0.2 | 43.4 ± 2.6 | 39.2 ± 0.8 | 36.2 ± 1.7 |
| Connectivity Density (1/mm3) | 389 ± 40 | 305 ± 25 | 181.7 ± 13 | 186.5 ± 35.1 | 173 ± 20 | 296 ± 103 |
| Structure Model Index | 1.2 ± 0.1 | 1.5 ± 0.1 | 0.6 ± 0.1 | 0.6 ± 0.2 | 0.7 ± 0.1 | 0.9 ± 0.2 |
Bone μCT was performed on vertebrae from 1, 3 and 6 month old male and female Grem1 null and wild type littermate controls. Voxel size used for analysis was 6 μm3. Values are means ± SEM; n = 4 to 6.
*
Significantly different from controls, p < 0.05 by unpaired t test.