Table 4.
Vertebral histomorphometry of 3 month old male and female Grem1 null mice and wild type littermate controls.
| Males | Wild Type | Grem1 null |
|---|---|---|
| Bone Volume/Tissue Volume (%) | 20.7 ± 0.9 | 10.7 ± 0.3* |
| Trabecular Separation (μm) | 157 ± 5 | 275 ± 9* |
| Trabecular Number (1/mm) | 5.1 ± 0.1 | 3.3 ± 0.1* |
| Trabecular Thickness (μm) | 40.6 ± 1.2 | 32.7 ± 0.6* |
| Osteoblast Surface/Bone Surface (%) | 17.7 ± 1.3 | 26.2± 2.5* |
| Number of Osteoblasts/Bone Perimeter (1/mm) | 15.5 ± 1.1 | 23.7 ± 2.3* |
| Number of Osteoblasts/Tissue Area (1/mm2) | 157 ± 9 | 153 ± 12 |
| Osteoclast Surface/Bone Surface (%) | 6.7 ± 0.6 | 7.2 ± 0.8 |
| Number of Osteoclasts/Bone Perimeter (1/mm) | 3.3 ± 0.3 | 3.6 ± 0.4 |
| Number of Osteoclasts/Tissue Area (1/mm2) | 33.5 ± 2.8 | 23.3 ± 2.8* |
| Eroded Surface/Bone Surface (%) | 15.1 ± 1.4 | 15.5 ± 1.8 |
| Mineral Apposition Rate (μm/day) | 1.11 ± 0.06 | 1.05 ± 0.03 |
| Mineralizing Surface/Bone Surface (%) | 2.9 ± 0.3 | 5.7 ± 0.9* |
| Bone Formation Rate (μm3/μm2/day) | 0.033± 0.004 | 0.061 ± 0.02* |
| Females | Wild Type | Grem1 null |
| Bone Volume/Tissue Volume (%) | 15.4 ± 0.5 | 15.0 ± 3.0 |
| Trabecular Separation (μm) | 218 ± 11 | 246 ± 67 |
| Trabecular Number (1/mm) | 3.9 ± 0.2 | 4.0 ± 0.6 |
| Trabecular Thickness (μm) | 39.3 ± 0.9 | 36.7 ± 2.3 |
| Osteoblast Surface/Bone Surface (%) | 26.1 ± 1.5 | 26.4 ± 4.5 |
| Number of Osteoblasts/Bone Perimeter (1/mm) | 22.4 ± 1.4 | 23.1 ± 4.0 |
| Number of Osteoblasts/Tissue Area (1/mm2) | 176 ± 13 | 176 ± 35 |
| Osteoclast Surface/Bone Surface (%) | 9.6 ± 1.1 | 8.5 ± 0.7 |
| Number of Osteoclasts/Bone Perimeter (1/mm) | 4.7 ± 0.5 | 4.1 ± 0.4 |
| Number of Osteoclasts/Tissue Area (1/mm2) | 36.5± 3.0 | 33.6 ± 7.5 |
| Eroded Surface/Bone Surface (%) | 21.2 ± 2.6 | 17.9 ± 1.8 |
Bone histomorphometry was performed on Lumbar 3 vertebrae from 3 month old male and female Grem1 null and wild type littermate controls. Values are means ± SEM; n = 4 to 6.
*
Significantly different from controls p < 0.05 by unpaired t test. Dual calcein and demeclocycline labels were present only in 2 control vertebrae from female mice, not allowing proper histomorphometric analysis.